A comprehensive review of genomic-scale genetic engineering as a strategy to improve bacterial productivity

Mario Alberto Pantoja Alonso, José Alberto Camas Reyes, Rafael Cano Segura, María del Rosario Cárdenas Aquino and Agustino Martínez Antonio

Te invitamos a leer el artículo "A comprehensive review of genomic-scale genetic engineering as a strategy to improve bacterial productivity" publicado en "Microbiology Society" en el que colaboró el Dr. Agustino Martínez Antonio de Cinvestav Irapuato.

Autores:

Mario Alberto Pantoja Alonso, José Alberto Camas Reyes, Rafael Cano Segura, María del Rosario Cárdenas Aquino and Agustino Martínez Antonio

Resumen:

Bacterial genome engineering has evolved to provide increasingly precise, robust and rapid tools, driving the development and optimization of bacterial production of numerous compounds. The field has progressed from early random mutagenesis methods, labour-intensive and inefficient, to rational and multiplexed strategies enabled by advances in genomics and synthetic biology. Among these tools, CRISPR/Cas has stood out for its versatility and its ability to achieve precision levels ranging from 50% to 90%, compared to the 10–40% obtained with earlier techniques, thereby enabling remarkable improvements in bacterial productivity. Nevertheless, like its predecessors, it still demands continuous refinement to reach full maturity. In this context, the present review addresses the lack of a unified overview by summarizing historical milestones and practical applications of genomic engineering tools in bacteria. It integrates diverse approaches to provide a comprehensive perspective on the evolution and prospects of these fundamental biotechnological tools.