A NanoLuc-Based Transactivation Assay in Plants
15 de agosto
Te invitamos a leer el artículo "A NanoLuc-Based Transactivation Assay in Plants" publicado en Methods in Molecular Biology, Springer, a cargo del profesor investigador Dr. Stefan de Folter y su equipo de trabajo de la UGA-Langebio.
Autores: Rosa Esmeralda Becerra-García, José Erik Cruz-Valderrama, Vincent E. Cerbantez-Bueno, Nayelli Marsch-Martínez & Stefan de Folter
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Unidad de Genómica Avanzada (UGA-Langebio), CINVESTAV-IPN.
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Unidad Irapuato, CINVESTAV-IPN.
Felicitamos al estudiantado y profesorado que contribuyeron en esta investigación por su arduo trabajo.
Abstract:
- Protein–DNA interactions are determinant of the regulation of gene expression in living organisms. Luminescence studies have been used in a wide range of techniques to identify how gene transcription can be regulated by proteins such as transcription factors (TFs). Despite the great advances in the use of luciferases as reporters in the performance of this mechanism, some of them still have disadvantages that have been tried to be solved by the generation of new luciferases that induce a more stable and perfectly visualizable reaction. NanoLuc is a recently described luciferase that has been characterized by its efficient, stable, and powerful luminescence. These qualities have been considered to create a new and efficient reporter system to detect protein–DNA interactions. In this chapter, we take advantage of NanoLuc and describe its use in a reliable procedure to detect protein–DNA interactions in Nicotiana benthamiana extracts and entire leaves.
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